Blood & Blood Circulation - Part 2

 BLOOD & BLOOD CIRCULATION

PART 2

COAGULATION OF BLOOD

 
•    Blood exhibits coagulation or clotting in response to an injury or trauma. This is a mechanism to prevent excessive loss of blood from the body.
•    Dark reddish brown scum is formed at the site of a cut or an injury over a period of time. It is a clot or coagulam formed mainly of a network of threads called fibrins in which dead and damaged formed elements of blood are trapped.
•    Fibrins are formed by the conversion of inactive fibrinogens in the plasma by the enzyme thrombin.
•    Thrombins, in turn are formed from another inactive substance present in the plasma called prothrombin. An enzyme complex, thrombokinase, is required for the above reaction. This complex is formed by a series of linked enzymic reactions (cascade process) involving a number of factors present in the plasma in an inactive state.
•    An injury or a trauma stimulates the platelets in the blood to release certain factors which activate the mechanism of coagulation. Certain factors released by the tissues at the site of injury also can initiate coagulation. Calcium ions play a very important role in clotting.

LYMPH (TISSUE FLUID)
•    As the blood passes through the capillaries in tissues, some water along with many small water soluble substances move out into the spaces between the cells of tissues leaving the larger proteins and most of the formed elements in the blood vessels. This fluid released out is called the interstitial fluid or tissue fluid.
•    Interstitial fluid or tissue fluid has the same mineral distribution as that in plasma. Exchange of nutrients, gases, etc., between the blood and the cells always occur through this fluid.
•    An elaborate network of vessels called the lymphatic system collects this fluid and drains it back to the major veins. The fluid present in the lymphatic system is called the lymph.
•    Lymph is a colourless fluid containing specialized lymphocytes which are responsible for the immune responses of the body. Lymph is also an important carrier for nutrients, hormones, etc.
•    Fats are absorbed through lymph in the lacteals present in the intestinal villi.

BLOOD GROUPS
•    As you know, blood of human beings differ in certain aspects though it appears to be similar. Various types of grouping of blood has been done. Two such groupings – the ABO and Rh – are widely used all over the world.

1.    ABO grouping
•    ABO grouping is based on the presence or absence of two surface antigens (chemicals that can induce immune response) on the RBCs namely A and B.
•    Similarly, the plasma of different individuals contain two natural antibodies (proteins produced in response to antigens).
•    The distribution of antigens and antibodies in the four groups of blood, A, B, AB and O are given in Table below.
•    ABO blood groups are controlled by the gene I. The plasma membrane of the red blood cells has sugar polymers that protrude from its surface and the kind of sugar is controlled by the gene. The gene (I) has three alleles IA, IB and i.
•    The alleles IA and IB produce a slightly different form of the sugar while allele i does not produce any sugar.

•    Because humans are diploid organisms, each person possesses any two of the three I gene alleles.
•    IA and IB are completely dominant over i, in other words when IA and i are present only IA expresses (because i does not produce any sugar), and when IB and i are present IB expresses.
•    But when IA and IB are present together they both express their own types of sugars: this is because of co-dominance. Hence red blood cells have both A and B types of sugars.
•    Since there are three different alleles, there are six different combinations of these three alleles that are possible, and therefore, a total of six different genotypes of the human ABO blood types. How many phenotypes are possible?
•    Here there are 6 Genotypes and 4 Phenotypes [A, B, AB and O]. [Inheritance – Mendel’s Laws of Inheritance]

•    You probably know that during blood transfusion, any blood cannot be used; the blood of a donor has to be carefully matched with the blood of a recipient before any blood transfusion to avoid severe problems of clumping (destruction of RBC).

•    From the above mentioned table it is evident that group ‘O’ blood can be donated to persons with any other blood group and hence ‘O’ group individuals are called ‘universal donors’.
•    Persons with ‘AB’ group can accept blood from persons with AB as well as the other groups of blood. Therefore, such persons are called ‘universal recipients’.

2.    Rh grouping
•    Another antigen, the Rh antigen similar to one present in Rhesus monkeys (hence Rh), is also observed on the surface of RBCs of majority (nearly 80 per cent) of humans. Such individuals are called Rh positive (Rh+ve) and those in whom this antigen is absent are called Rh negative (Rh-ve).
•    An Rh-ve person, if exposed to Rh+ve blood, will form specific antibodies against the Rh antigens. Therefore, Rh group should also be matched before transfusions.
•    A special case of Rh incompatibility (mismatching) has been observed between the Rh-ve blood of a pregnant mother with Rh+ve blood of the foetus.
•    Rh antigens of the foetus do not get exposed to the Rh-ve blood of the mother in the first pregnancy as the two bloods are well separated by the placenta.
•    However, during the delivery of the first child, there is a possibility of exposure of the maternal blood to small amounts of the Rh+ve blood from the foetus.
•    In such cases, the mother starts preparing antibodies against Rh antigen in her blood.
•    In case of her subsequent pregnancies, the Rh antibodies from the mother (Rh-ve) can leak into the blood of the foetus (Rh+ve) and destroy the foetal RBCs.
•    This could be fatal to the foetus or could cause severe anaemia and jaundice to the baby. This condition is called erythroblastosis foetalis.
•    This can be avoided by administering anti-Rh antibodies to the mother immediately after the delivery of the first child